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Related Experiment Videos

The African trypanosome genome.

N M El-Sayed1, P Hegde, J Quackenbush

  • 1The Institute for Genomic Research (TIGR), 9712 Medical Center Drive, Rockville, MD 20850, USA. nelsayed@tigr.org

International Journal for Parasitology
|March 25, 2000
PubMed
Summary
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The African trypanosome genome sequencing reveals extensive chromosome size variation and gene organization. Microarray analysis demonstrates its utility in identifying differentially expressed genes across parasite life stages.

Area of Science:

  • Genomics
  • Parasitology
  • Molecular Biology

Background:

  • The African trypanosome, Trypanosoma brucei, possesses a complex genome with significant size variation.
  • Its DNA is organized into megabase chromosomes, intermediate chromosomes, and minichromosomes.
  • Telomeres contain TTAGGG repeats and are linked to variant surface glycoprotein (VSG) genes.

Purpose of the Study:

  • To characterize the genome structure and organization of Trypanosoma brucei.
  • To explore the role of minichromosomes in VSG gene storage.
  • To assess the applicability of microarray technology for gene expression analysis in T. brucei.

Main Methods:

  • Whole-genome sequencing and analysis of DNA fragments.
  • Characterization of chromosomal structure and telomeric regions.

Related Experiment Videos

  • Development and application of a cDNA microarray for gene expression profiling.

    • Main Results:

    • Discontinuous genomic sequence data exceeding 20 Mb has been generated.
    • Complete sequences for chromosomes I and II are being determined.
    • Microarray analysis successfully identified differentially expressed genes between developmental stages.

    Conclusions:

    • The Trypanosoma brucei genome exhibits substantial size variation and a unique organization of VSG genes.
    • Microarray technology is a valuable tool for studying gene expression in T. brucei.
    • Ongoing sequencing efforts are expected to yield the complete genome sequence soon.