Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expanded bed adsorption on supermacroporous cross-linked cellulose matrix.

A Pai1, S Gondkar, S Sundaram

  • 1Department of Chemical Technology, University of Mumbai, Matunga, India.

Bioseparation
|March 29, 2000
PubMed
Summary

Cellulose-based adsorbent beads (CELBEADS) show promise for expanded bed chromatography, offering efficient purification of lactate dehydrogenase with high yield and reusability. These beads outperform commercial matrices in chromatography performance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026
Same author

III-Nitride MEMS drum resonators on flexible metal substrates.

Microsystems & nanoengineering·2025
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025
Same author

Disease and treatment awareness, prescription patterns, and self-medication practices in patients of functional bowel disorders: A cross-sectional survey.

Journal of postgraduate medicine·2025
Same author

Impact of Asana and Pranayama on Heart Rate Variability and Cardiac Autonomic Functions in Young Femaleswith Premenstrual Syndrome: An Interventional Clinical Trial.

La Clinica terapeutica·2025
Same author

Natural occurrence of episomal banana streak GF virus species infecting the GI-tagged banana cv. Virupakshi in India.

Physiology and molecular biology of plants : an international journal of functional plant biology·2025

Area of Science:

  • Biochemistry
  • Chromatography
  • Materials Science

Background:

  • Expanded bed chromatography (EBC) is crucial for direct purification of biomolecules from complex matrices.
  • Developing novel, high-performance adsorbent matrices is essential for improving EBC efficiency.
  • Cellulose-based materials offer potential as sustainable and adaptable chromatography supports.

Purpose of the Study:

  • To characterize rigid spherical macroporous adsorbent beads (CELBEADS) derived from cellulose.
  • To evaluate CELBEADS as a matrix for expanded bed affinity chromatography.
  • To compare the chromatographic performance of CELBEADS against a commercial matrix and demonstrate its utility in enzyme purification.

Main Methods:

  • CELBEADS were prepared via cellulose cross-linking.

Related Experiment Videos

  • Chromatographic performance was assessed using tyrosine, papain, and bovine serum albumin, measuring height equivalent to theoretical plate (HETP).
  • Lactate dehydrogenase purification from porcine muscle homogenate was performed using Cibacron blue-conjugated CELBEADS in both packed and expanded bed modes.

    • Main Results:

    • CELBEADS exhibited HETP profiles typical of macroporous adsorbents, showing improvement with velocity.
    • CELBEADS yielded lower HETP values for papain and bovine serum albumin compared to a commercial Streamline DEAE matrix.
    • Purification of lactate dehydrogenase achieved a 14-fold purification in approximately 60 minutes, with successful repeated use after cleaning.

    Conclusions:

    • Rigid spherical macroporous cellulose beads (CELBEADS) are suitable for expanded bed affinity chromatography.
    • CELBEADS demonstrate superior or comparable performance to commercial matrices.
    • The developed CELBEADS matrix is effective for enzyme purification and exhibits good reusability and cleanability.