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Related Experiment Videos

Characterisation of STREAMLINE phenyl.

J Färenmark1, J Gustavsson, I Lagerlund

  • 1Amersham Pharmacia Biotech AB, Uppsala, Sweden.

Bioseparation
|March 29, 2000
PubMed
Summary

STREAMLINE Phenyl, a new chromatography support for expanded bed adsorption, demonstrates excellent chemical and chromatographic stability across a wide pH range and temperatures. Its hydrophobic interaction properties ensure retained performance after storage, highlighting its robustness for bioprocessing applications.

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Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Separation Science

Background:

  • Hydrophobic interaction chromatography (HIC) is crucial for protein purification.
  • Expanded bed adsorption (EBA) allows direct processing of unclarified feedstocks.
  • Development of robust HIC supports for EBA is essential for efficient bioseparation.

Purpose of the Study:

  • To evaluate the chemical and chromatographic stability of STREAMLINE Phenyl for expanded bed adsorption.
  • To assess the breakthrough capacity of STREAMLINE Phenyl for human IgG.
  • To investigate the impact of bed height on dynamic capacity in EBA.

Main Methods:

  • Chemical stability assessed by monitoring carbon leakage (Total Organic Carbon) from the matrix across pH 1-14 at 20°C and 40°C.

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  • Chromatographic stability evaluated after 12-week storage in various solutions, followed by a functional test.
  • Breakthrough capacity determined for human IgG, analyzing the effect of bed height in packed and expanded beds.

    • Main Results:

    • STREAMLINE Phenyl exhibited high chemical stability with minimal carbon leakage under tested conditions.
    • The adsorbent retained its chromatographic properties after prolonged storage in different solutions.
    • Breakthrough capacity was significantly influenced by bed height, with good correlation between packed and expanded bed data.

    Conclusions:

    • STREAMLINE Phenyl is a chemically and chromatographically stable HIC support suitable for EBA.
    • The study confirms the importance of bed height for optimizing dynamic capacity in EBA processes.
    • Results support the use of STREAMLINE Phenyl for robust and efficient bioseparation applications.