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A simplified purification procedure of alpha-lactalbumin from milk using Ca(2+)-dependent adsorption in hydrophobic
W Noppe1, P Haezebrouck, I Hanssens
1Interdisciplinary Research Centre, Katholieke Universiteit Leuven, Kortrijk, Belgium. wim.noppe@kulak.ac.be
Abstract:
The technique of expanded bed adsorption is originally designed for a direct recovery of proteins from fermentor feedstocks. In this article we describe the use of expanded bed adsorption for the recovery of alpha-lactalbumins from defatted milk using the hydrophobic Streamline Phenyl gel. alpha-Lactalbumins are Ca(2+)-binding proteins. Upon Ca2+ removal, they undergo a significant conformation change rendering them more hydrophobic. Based on this unique property we develop a protocol for fast and efficient purification of alpha-lactalbumin from milk. The use of this technique results in a reduction of the number of chromatographic purification steps.