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Related Experiment Videos

[Programmed cell death (apoptosis) in excised subretinal neovascularization].

R Wiezorrek1, H Schäfer, A A Bialasiewicz

  • 1Augenklinik, Universitäts-Krankenhaus, Hamburg-Eppendorf.

Der Ophthalmologe : Zeitschrift Der Deutschen Ophthalmologischen Gesellschaft
|March 29, 2000
PubMed
Summary

Apoptosis, or programmed cell death, is common in choroidal neovascularizations (CNV), especially in recent cases of age-related macular degeneration (AMD). Its presence suggests that treatment timing is crucial for successful outcomes in CNV therapy.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Pathology

Background:

  • Choroidal neovascularizations (CNV) are a leading cause of vision loss, particularly in age-related macular degeneration (AMD).
  • The role and extent of apoptosis (programmed cell death) in CNV development and progression are not well understood.
  • Investigating apoptosis in CNV may reveal new therapeutic strategies.

Purpose of the Study:

  • To analyze the incidence and distribution of apoptosis in surgically excised CNV membranes.
  • To correlate the presence of apoptosis with the duration of CNV and clinical presentation in patients with AMD.
  • To explore the potential therapeutic implications of apoptosis in CNV.

Main Methods:

  • Surgical excision of 34 CNV membranes from patients with various conditions, including AMD, post-traumatic proliferative vitreoretinopathy, and penetrating eye injuries.

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  • Histological examination using Periodic Acid-Schiff (PAS) and Hematoxylin and Eosin (H&E) staining.
  • Immunohistochemical detection of apoptotic cells using the in situ cell-detection kit (TUNEL) assay.

    • Main Results:

    • Apoptotic cells were detected in 74% of all CNV samples and 76% of AMD-related CNV samples.
    • Apoptosis was more prevalent in recent CNV (existing 1-12 months) compared to long-standing lesions (existing 5-36 months).
    • In AMD patients, the duration of vision loss was shorter in those with detectable apoptosis.

    Conclusions:

    • Programmed cell death is an active mechanism in CNV tissue with variable involvement.
    • A correlation exists between the duration of CNV and the incidence of apoptosis in AMD patients.
    • The findings suggest that the timing of intervention may significantly impact treatment success for CNV.