Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro fertilization with flow-cytometrically-sorted bovine sperm.

K H Lu1, D G Cran, G E Seidel

  • 1XY, Inc., Fort Collins, CO 80523, USA.

Theriogenology
|March 29, 2000
PubMed
Summary

In vitro fertilization (IVF) with sexed sperm shows similar fertilization rates but reduced blastocyst production in cattle. Further research is needed to optimize IVF protocols for sexed sperm to improve embryo development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of breast cancer history on decision-making for ovarian cancer risk-reducing surgery in the TUBA-WISP-II study.

American journal of obstetrics and gynecology·2025
Same author

[Early results of modified Bikini approach periacetabular osteotomy for the treatment of developmental hip dysplasia under 50 years of age].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2023
Same author

Applications and world-wide use of sexed semen in cattle.

Animal reproduction science·2021
Same author

Pre-synchronization of ovulation timing and delayed fixed-time artificial insemination increases pregnancy rates when sex-sorted semen is used for insemination of heifers.

Animal reproduction science·2021
Same author

Two split-time artificial insemination programs in suckled beef cows.

Journal of animal science·2018
Same author

Phase I-II clinical trial design: a state-of-the-art paradigm for dose finding.

Annals of oncology : official journal of the European Society for Medical Oncology·2017

Area of Science:

  • Animal Science
  • Reproductive Biology
  • Biotechnology

Background:

  • In vitro fertilization (IVF) is a key technology in animal breeding.
  • Sperm sexing technologies, like flow cytometry, offer potential for selective breeding.
  • Sexed sperm may require modified IVF protocols due to pre-capacitation.

Purpose of the Study:

  • To evaluate the efficacy of IVF using sexed bovine sperm.
  • To compare early embryonic development and blastocyst production rates between sexed and unsexed sperm.
  • To assess the viability of using sexed sperm in conjunction with IVF for cattle breeding.

Main Methods:

  • IVF procedures were performed using frozen, sexed bovine sperm and compared to unsorted sperm.
  • Fertilization rates, early cell cycle events, and blastocyst development were monitored.

Related Experiment Videos

  • The potential impact of sperm sorting on embryo quality and developmental timing was assessed.

    • Main Results:

    • Fertilization rates and early cell cycle timing were comparable between sexed and unsorted sperm.
    • Blastocyst production was reduced by approximately 30% when using sexed sperm compared to controls.
    • Some studies indicated a slight delay in blastocyst development with sexed sperm.

    Conclusions:

    • While sexed sperm can achieve fertilization, current IVF protocols need optimization for improved blastocyst development in cattle.
    • IVF with sexed sperm has demonstrated success in producing viable, sex-preselected calves.
    • Further advancements in sperm sorting and IVF techniques are expected to increase the utilization of sexed sperm in cattle breeding programs.