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A low-voltage droplet charging circuit with simulative cell-sorting function for flow cytometer-cell sorter.

H Zhou¹, B Lin, W Wu

¹Institute of Biophysics, Chinese Academy of Sciences, Beijing, People's Republic of China. zhw@bptechlin.ibp.ac.cn

|March 30, 2000

Summary

Researchers developed a low-voltage charging circuit for flow cytometer cell sorters, offering an affordable and accessible alternative to expensive high-voltage systems for efficient cell separation.

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Area of Science:

Biotechnology
Analytical Chemistry

Background:

Flow cytometer cell sorters are essential in biological research.
Commercial cell sorters often rely on expensive, hard-to-obtain high-voltage amplifiers for droplet deflection.
This necessitates the development of more accessible alternatives.

Purpose of the Study:

To design and implement a cost-effective, low-voltage droplet charging circuit for flow cytometer cell sorters.
To provide an alternative to expensive high-voltage systems, making cell sorting technology more accessible.

Main Methods:

A novel low-voltage charging circuit was designed.
Complementary positive and negative charging pulses were generated for cell separation.
Two low-voltage amplifiers boosted these pulses to drive charging electrodes.

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Main Results:

The circuit achieved double the voltage through electrode addition.
Cell sorting efficiency with the low-voltage system matched that of high-voltage systems.
A simulative cell-sorting function was successfully implemented.

Conclusions:

The developed low-voltage charging circuit is a viable, cost-effective alternative to high-voltage systems.
This technology is particularly beneficial for researchers building personal cell sorting instruments.
It offers an easier-to-build and more affordable solution for cell separation.