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Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Published on: October 18, 2016
The human dendritic cell marker CD83 maps to chromosome 6p23
S Berchtold1, T Jones, P Mühl-Zürbes
1Department of Dermatology, University of Erlangen-Nürnberg, Germany.
Annals of Human Genetics
|March 30, 2000
Summary
The human CD83 gene was precisely mapped to chromosome 6 band p23 using advanced genetic mapping techniques. This finding aids in understanding gene location for further research and potential therapeutic targets.
Area of Science:
- Genetics
- Human Molecular Biology
- Chromosomal Gene Mapping
Background:
- The CD83 gene plays a role in immune regulation.
- Accurate chromosomal localization is crucial for genetic studies and disease association.
Purpose of the Study:
- To determine the specific chromosomal location of the human CD83 gene.
- To refine the gene map for chromosome 6.
Main Methods:
- Somatic cell hybrid analysis
- Radiation hybrid mapping (Genebridge4 panel)
- Fluorescence In Situ Hybridization (FISH) on human metaphase chromosomes
Main Results:
- PCR analysis confirmed CD83 on human chromosome 6.
- Radiation hybrid mapping placed CD83 between markers AFMa192wg9 and AFMb322wd1 (lod score 9.2).
- FISH analysis localized the CD83 gene to chromosome 6 band p23.
Conclusions:
- The human CD83 gene is definitively located at chromosome 6p23.
- This precise localization provides a foundation for future functional and positional cloning studies.
