Bone marrow processing using the fenwal CS-3000 plus blood cell separator: results of 99 procedures

Area of Science:

• Hematology
• Cellular Therapy
• Biotechnology

Background:

• Bone marrow transplantation (BMT) is a crucial therapy for various malignant and nonmalignant diseases.
• Effective bone marrow (BM) processing is a prerequisite for advanced ex vivo treatment techniques.
• The Fenwal CS-3000 Plus cell separator is utilized for BM processing.

Purpose of the Study:

• To evaluate the efficacy of the Fenwal CS-3000 Plus cell separator for bone marrow processing.
• To assess cell recovery, purity, and volume reduction in processed bone marrow.
• To compare processing outcomes between pediatric and adult patients.

Main Methods:

• Processing of 99 bone marrow samples using the Fenwal CS-3000 Plus cell separator with a specialized program.
• Quantification of nucleated cells (NC), mononuclear cells (MNC), red blood cells (RBC), platelets, colony-forming units-granulocyte-macrophage (CFU-GM), CD34+ cells, and volume.
• Analysis of relative purity of MNC and polymorphonuclear cells (PMN).

Main Results:

• High recovery rates for MNC (82.2%), CFU-GM (117.6%), and CD34+ cells (97.8%).
• Significant removal of RBC (96%), PMN (92%), platelets (37.2%), and volume (85.1%).
• Lower volume reduction in pediatric patients compared to adults (79.6% vs. 88.8%), with no other significant differences.

Conclusions:

• The Fenwal CS-3000 Plus cell separator is effective for bone marrow processing, yielding a product suitable for ex vivo manipulation.
• The processing method demonstrates high efficiency in removing unwanted components while preserving critical cell populations.
• The system is applicable to both pediatric and adult bone marrow processing, with minor volume reduction differences in pediatric cases.