Storage-associated physicochemical changes in extracellular vesicles derived from human mesenchymal stromal cells

Area of Science:

• Biomedical Engineering
• Cell Biology
• Regenerative Medicine

Background:

• Extracellular vesicles (EVs) from mesenchymal stromal cells (MSCs) are promising therapeutic agents due to their signaling capabilities.
• Standardized manufacturing and storage protocols are crucial for ensuring the quality and efficacy of EV-based products.

Purpose of the Study:

• To evaluate the physicochemical stability of MSC-derived EVs under different storage temperatures.
• To assess the impact of storage conditions on EV properties for therapeutic applications.

Main Methods:

• MSCs were isolated from adipose tissue, umbilical cord, and dental pulp.
• EVs were isolated using differential ultracentrifugation and characterized by NTA and ELISA.
• Physicochemical stability was assessed at +4°C to +6°C, -20°C, and -80°C over time.

Main Results:

• EV particle and protein concentrations remained stable for at least 14 days at +4°C to +6°C.
• EVs stored at -20°C were stable for at least 6 months, and at -80°C for at least 10 months.
• Storage conditions did not compromise key EV characteristics, including bFGF content.

Conclusions:

• MSC-derived EVs exhibit physicochemical stability during long-term storage in saline solution.
• These findings support the clinical development of MSC-derived EV-based therapeutic products.