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[Ara-c induced apoptosis in human myeloid leukemia cell line HL-60]
Objective:
To elucidate the pattern of chemotherapy drugs induced apoptosis and its role in chemotherapy of acute leukemia.
Methods:
Apoptosis induced by Ara-c in human myeloid leukemia cell line HL-60 was investigated by applying light microscopy, electron microscopy combined with DNA electrophoresis and flow cytometry analysis techniques.
Results:
Apoptosis persisted throughout 36 hours following addition of Ara-c with a gradual augmentation. Efficiency of apoptosis was enhanced in a dose-dependent pattern, HL-60 treated with six other chemotherapy drugs and perpherial white blood cells from a AML case undergoing DA protocol chemotherapy exhibited typical DNA ladder pattern. Further investigation indicated that chemotherapy drugs induced apoptosis came into being possibly by downregulation the oncogenes expression of c-myc and bcl-2.
Conclusion:
Chemotherapy induced apoptosis is a primary mechanism in chemotherapy.