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Trypanocidal activity of organotin chlorides on Trypanosoma brucei-infected mice
M N Shuaibu1, D A Ameh, J J Bonire
1Institute of Tropical Medicine, Nagasaki University, Japan.
Abstract:
The organotin compounds dibutyltin (DBTC) and diphenyltin dichlorides (DPTC) were tested for trypanocidal activity on a Trypanosoma brucei-infected mice model. At a dose of 10 mg DBTC and 15 mg DPTC/kg/day for five consecutive days, they cleared the parasites from the peripheral blood of the infected mice. Subinoculation of some healthy mice with the homogenates of liver, spleen, kidney, cerebrospinal fluid and blood from the mice considered cured, showed a few cases of relapse. The LD50 of DBTC and DPTC are 90 mg/kg and 75 mg/kg respectively.
Insights
Organotin compounds dibutyltin (DBTC) and diphenyltin dichlorides (DPTC) showed trypanocidal activity against Trypanosoma brucei in mice. While clearing peripheral blood parasites, some relapses occurred, indicating further research is needed.
Area of Science:
- Medicinal Chemistry
- Parasitology
- Toxicology
Background:
- Trypanosomiasis is a significant parasitic disease.
- Organotin compounds are being investigated for antiparasitic properties.
Purpose of the Study:
- To evaluate the trypanocidal activity of dibutyltin (DBTC) and diphenyltin dichlorides (DPTC).
- To assess the efficacy and toxicity of these compounds in a Trypanosoma brucei mouse model.
Main Methods:
- DBTC and DPTC were administered to Trypanosoma brucei-infected mice.
- Parasite clearance in peripheral blood was monitored.
- Relapse rates were assessed through subinoculation.
- Lethal dose 50 (LD50) was determined for both compounds.
Main Results:
- DBTC (10 mg/kg/day) and DPTC (15 mg/kg/day) cleared parasites from peripheral blood in infected mice after five days.
- A few cases of relapse were observed in cured mice.
- The LD50 values were 90 mg/kg for DBTC and 75 mg/kg for DPTC.
Conclusions:
- DBTC and DPTC exhibit significant trypanocidal activity.
- Further investigation is required to address the observed relapses and optimize treatment strategies.
- The toxicity profiles (LD50) suggest a need for careful dosage considerations.