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Processing of mRNA from human leukocytes by biomagnetical separation: comparison with current methods of RNA
1Department of Clinical Immunology, Medical Faculty of Palacký University, Olomouc, Czech Republic.
Acta Universitatis Palackianae Olomucensis Facultatis Medicae
|April 1, 2000
Abstract:
The article refers to the technique of biomagnetical separation (BS) of mRNA using "Dynabeads mRNA DIRECT". After a brief survey of RNA isolation methods authors describe a general procedure of BS as well as its concrete application for mRNA isolation from bronchoalveolar cells. Authors report results of specific experiments to assess the efficiency and accuracy of mRNA isolation by BS. The applicability of BS technique for the gene expression studies is discussed in the end of the article.