Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ANTIPHOSPHOLIPID SYNDROME AND MONOCYTES: NEW ASPECTS.

A Blbulyan1, A Martirosyan1, M Petrek1

  • 1Palacky University, Faculty of Medicine and Dentistry, Department of Pathological Physiology, Czech Republic; Institute of Molecular Biology, National Academy of Sciences, Group of Molecular and Cellular Immunology, Yerevan; Institute of Perinatology, Obstetrics and Gynecology, Department of Obstetrics, Yerevan, Armenia.

Georgian Medical News
|August 19, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Angiogenic imbalance and endothelial dysfunction in the placenta in antiphospholipid syndrome.

Autoimmunity reviews·2026
Same author

Impact of antiphospholipid syndrome on placenta and uterine NK cell function: insights from a mouse model.

Scientific reports·2024
Same author

Traumatic brain injury promotes neurogenesis at the cost of astrogliogenesis in the adult hippocampus of male mice.

Nature communications·2024
Same author

Association between sarcoidosis and HLA polymorphisms in a Czech population from Central Europe: focus on a relationship with clinical outcome and treatment.

Frontiers in medicine·2023
Same author

Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection.

Osteoarthritis and cartilage·2022
Same author

TP53 rs1042522 and rs8064946 variants in myocardial infarction.

Bratislavske lekarske listy·2019
Same journal

NON-PHARMACOLOGICAL INTERVENTIONS FOR RESTLESS LEG SYNDROME IN HEMODIALYSIS PATIENTS: A SYSTEMATIC REVIEW AND NETWORK META-ANALYSIS.

Georgian medical news·2026
Same journal

CLINICAL RESULTS OF DELORME'S AND ALTEMEIER'S PROCEDURES IN RECTAL PROLAPSE.

Georgian medical news·2026
Same journal

EVALUATION OF GEORGIAN MEDICAL DOCTORS' RESEARCH EXPERIENCE AND PERCEPTIONS TOWARD COLLABORATIVE RESEARCH WITH UNIVERSITIES.

Georgian medical news·2026
Same journal

PSYCHOLOGICAL ASPECTS OF USING SUGGESTIVE METHODS IN COGNITIVE-BEHAVIORAL THERAPY.

Georgian medical news·2026
Same journal

MORPHOLOGY AND PREVALENCE OF C-SHAPED CANALS IN MANDIBULAR FIRST MOLARS OF AN IRAQI KURDISTAN REGION POPULATION: A CONE-BEAM COMPUTED TOMOGRAPHY ASSESSMENT.

Georgian medical news·2026
Same journal

DERMATOSURGERY ROUNDS: THE DOUBLE ROTATION (YIN-YANG) FLAP AS BASIC WEAPON IN THE FIGHT AGAINST KERATINOCYTE CANCER OF THE SCALP.

Georgian medical news·2026
See all related articles

Monocytes from women with antiphospholipid syndrome (APS) show heightened inflammatory responses to lipopolysaccharide (LPS). ATP can reduce this LPS-induced inflammation, suggesting a potential regulatory mechanism for APS monocytes.

Area of Science:

  • Immunology
  • Molecular Biology
  • Pathogenesis of Autoimmune Diseases

Background:

  • Antiphospholipid syndrome (APS) pathogenesis involves monocytes, but their activation mechanisms remain unclear.
  • Monocyte activation is implicated in both thrombotic and non-thrombotic APS complications.

Purpose of the Study:

  • To investigate the transcriptional activity of monocytes from APS patients upon stimulation with lipopolysaccharide (LPS) and LPS+ATP.
  • To identify molecular pathways involved in monocyte activation in APS.

Main Methods:

  • Comparative RT-PCR analysis of monocyte gene expression.
  • Study included eleven women with APS and recurrent miscarriages and nine healthy women as controls.
  • Monocytes were exposed to low concentrations of LPS and LPS+ATP.

Related Experiment Videos

Main Results:

  • APS monocytes exhibited elevated mRNA levels of TLR2, IL-23, CCL2, CXCL10, IL-1β, and IL-6 after LPS stimulation.
  • Healthy monocytes showed increased IL-6 and STAT3 mRNA with LPS.
  • Dual stimulation (LPS+ATP) decreased CCL-2, IL-1β, and NLRP3 mRNA in APS cells but elevated TLR2 mRNA in both groups.

Conclusions:

  • Increased monocyte sensitivity to LPS in APS may contribute to thrombus formation.
  • Low concentrations of ATP may mitigate the LPS-induced inflammatory state in APS monocytes, indicating a potential regulatory pathway.