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Preparation, evaluation and application of new pseudo-affinity chromatographic supports for penicillin acylase
X Santarelli1, V Fitton, N Verdoni
1Ecole Supérieure de Technologie des Biomolécules de Bordeaux, Université Victor Segalen Bordeaux 2, France. xavier.santarelli@biomemb.u-bordeaux2.fr
Abstract:
New pseudo-affinity chromatographic supports for penicillin acylase were prepared and evaluated with three different samples: pure penicillin acylase, industrial clarified feedstock and crude extract. The different gels were studied for their purification fold (three to six) and their recovery power (80-100%). The best support was characterized by its dynamic capacity, (20 mg/ml) and its recovery power was tested at five flow-rates (30, 150, 300 and 750 cm/h) to determine the optimal flow-rate (300 cm/h). In addition we used cleaning in place to test the resistance to hard conditions of sanitization by 1 M NaOH (90% of recovery for 12 h of contact). These gels may therefore be used on an industrial scale.