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Molecular cloning and characterization of mouse CD97
J Hamann1, C van Zeventer, A Bijl
1Department of Immunobiology, CLB and Laboratory for Experimental and Clinical Immunology, Academic Medical Centre, University of Amsterdam, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands.
International Immunology
|April 1, 2000
Summary
Mouse CD97 (mCD97), a transmembrane receptor, binds to mouse CD55 (mCD55). This interaction is crucial for immune cell function and is phylogenetically restricted, with different species
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- The EGF-TM7 family, including CD97 and EMR1, comprises class II seven-span transmembrane receptors.
- These receptors are primarily expressed on immune system cells.
- CD55, a complement cascade regulator, was recently identified as a ligand for human CD97 (hCD97).
Purpose of the Study:
- To characterize the molecular properties of mouse CD97 (mCD97).
- To identify cellular ligands for mCD97.
- To investigate the phylogenetic specificity of the CD97-CD55 interaction.
Main Methods:
- Generation of mCD97 monoclonal antibodies (mAbs).
- Expression of mCD97 isoforms in COS cells.
- Erythrocyte and splenocyte adhesion assays.
- Inhibition of adhesion using mCD55 antibodies.
Main Results:
- mCD97 exhibits isoforms with three and four epidermal growth factor-like (EGF) domains (mCD97(EGF1,2,4) and mCD97(EGF1,2,3,4)), and a unique isoform with an inserted sequence (mCD97(EGF1,2,X,3,4)).
- mCD97 is expressed on lymphoid and myeloid cells, similar to hCD97.
- mCD55 was confirmed as a ligand for mCD97, mediating erythrocyte and splenocyte adhesion.
- Adhesion to mCD97 was dependent on specific EGF domain configurations, with the mCD97(EGF1,2,X,3,4) isoform showing no adhesion.
- The CD97-CD55 interaction is species-specific, with primate erythrocytes binding hCD97 and rodent erythrocytes binding mCD97.
Conclusions:
- Mouse CD55 is a cellular ligand for mouse CD97.
- The structure of the EGF domains in CD97 is critical for CD55 binding.
- The CD97-CD55 interaction is evolutionarily conserved within species groups but restricted across different species.