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Related Experiment Videos

Estimating recombinational parameters in Streptococcus pneumoniae from multilocus sequence typing data.

E J Feil1, J M Smith, M C Enright

  • 1Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, United Kingdom. ed.feil@ceid.ox.ac.uk

Genetics
|April 4, 2000
PubMed
Summary
This summary is machine-generated.

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Multilocus sequence typing (MLST) reveals that recombination is 10 times more frequent than mutation in generating new alleles in Streptococcus pneumoniae. This molecular typing method helps understand bacterial evolution and clonal divergence.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Evolutionary Genetics

Background:

  • Multilocus sequence typing (MLST) is a robust method for bacterial pathogen characterization.
  • MLST uses DNA sequences from housekeeping genes to differentiate bacterial isolates.
  • Streptococcus pneumoniae is a significant human pathogen with diverse genetic lineages.

Purpose of the Study:

  • To apply MLST to a large collection of Streptococcus pneumoniae isolates.
  • To investigate the relative roles of recombination and mutation in bacterial evolution.
  • To estimate the frequency and impact of recombination events in clonal divergence.

Main Methods:

  • Sequencing of approximately 450-bp internal fragments of seven housekeeping genes from 575 Streptococcus pneumoniae isolates.

Related Experiment Videos

  • Analysis of allelic profiles to define clonal complexes and single-locus variants.
  • Comparative sequence analysis to quantify recombination and mutation rates.

    • Main Results:

    • MLST identified distinct clonal complexes within the Streptococcus pneumoniae population.
    • Recombination was estimated to generate new alleles approximately 10-fold more frequently than mutation.
    • A single nucleotide site is approximately 50 times more likely to change via recombination than mutation.

    Conclusions:

    • MLST provides valuable insights into the population structure and evolutionary dynamics of Streptococcus pneumoniae.
    • Recombination plays a dominant role in generating genetic diversity and driving clonal divergence in this bacterium.
    • The study demonstrates a method for estimating the length of recombinational replacements using MLST data.