Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene-trap-based target site for cre-mediated transgenic insertion.

N Hardouin, A Nagy

    Genesis (New York, N.Y. : 2000)
    |April 5, 2000
    PubMed
    Summary

    This study presents an improved gene-trap method for efficiently studying gene expression patterns in mice. The new strategy allows for transgene insertion into trapped loci, linking transgene expression to reporter gene activity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A 1D Lyman-alpha profile camera for plasma edge neutral studies on the DIII-D tokamak.

    The Review of scientific instruments·2021
    Same author

    Absolute calibration of the Lyman-α measurement apparatus at DIII-D.

    The Review of scientific instruments·2021
    Same author

    Using metabolic markers to identify insulin resistance in premenopausal women with and without polycystic ovary syndrome.

    Journal of endocrinological investigation·2021
    Same author

    Subspace theory with spherically symmetric densities.

    The Journal of chemical physics·2021
    Same author

    Information theoretical and thermodynamic view of the excited-state density functional theory of Coulomb systems.

    The Journal of chemical physics·2020
    Same author

    Validating and assessing the oral health-related quality of life among Hungarian children with cleft lip and palate using Child-OIDP scale.

    European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2020

    Area of Science:

    • Developmental Biology
    • Genetics
    • Molecular Biology

    Background:

    • Studying tissue-specific gene expression in mice is crucial for understanding development and disease.
    • Traditional methods for identifying gene regulatory elements are often time-consuming and expensive.
    • Existing gene-trap strategies use reporters like LacZ or GFP to identify expression patterns of endogenous genes.

    Discussion:

    • This research introduces an enhanced gene-trap strategy for efficient transgene insertion.
    • The method utilizes Cre recombinase-mediated insertion into trapped genomic loci.
    • This approach links transgene expression to the reporter gene's regulatory elements.

    Key Insights:

    • An improved gene-trap strategy facilitates efficient Cre recombinase-mediated transgene insertion.
    • This post-integrational modification links transgene expression to the reporter's regulatory elements.
    • The method offers a more efficient way to study gene expression patterns.

    Outlook:

    • This technique can accelerate the study of gene function in mouse models.
    • It provides a valuable tool for dissecting gene regulatory networks.
    • Further applications in disease modeling and developmental studies are anticipated.

    Related Experiment Videos