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Allergens of Epicoccum nigrum grown in different media for quality source material.

V Bisht¹, B P Singh, N Arora

¹Centre for Biochemical Technology, Delhi University Campus, India.

|February 7, 2001

Summary

Epicoccum nigrum (EN) extract potency for allergy diagnosis varies with culture media. A 13-day culture in Sabouraud's broth with yeast extract (SBY) yielded the most potent EN extract for allergy testing.

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Area of Science:

Mycology
Immunology
Allergology

Background:

Epicoccum nigrum (EN) extract used for allergy disorders shows variability in protein composition and allergenic potency.
Investigating allergens from EN grown in different media is crucial for standardizing extracts.

Purpose of the Study:

To investigate the allergens of Epicoccum nigrum (EN) grown in various nutrient media.
To identify the optimal culture conditions for producing potent EN extracts for allergy diagnosis.

Main Methods:

EN was cultured in five different media for 5-23 days at 25°C.
Growth patterns were assessed by dry weight, protein, and carbohydrate content.
Antigenic and allergenic properties were evaluated using patient sera, rabbit antibodies, SDS-PAGE, IgE/IgG immunoblots, and ELISA.

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Main Results:

Sabouraud's broth with yeast extract (SBY) produced maximum spore-mycelial mass and protein content.
Potato dextrose broth (PDB) and agar (PDA) showed high dry weight and protein in 7-9 day cultures.
EN extract from a 13-day SBY culture exhibited the highest allergenic and antigenic activity, confirmed by IgE ELISA and intradermal tests.

Conclusions:

The 13-day culture of EN in SBY is the most potent.
This optimized EN extract can be selected for diagnostic purposes and future research in allergy.