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Spindle bodies of Heliothis armigera entomopoxvirus develop in structures associated with host cell endoplasmic

J Lai-Fook1, D J Dall

  • 1Division of Entomology, CSIRO, ACT, Canberra, 2601, Australia.

Journal of Invertebrate Pathology
|April 8, 2000
PubMed

Insights

The formation of spindle bodies in Heliothis armigera entomopoxvirus involves protein crystallization within infected cells. The chaperone BiP associates with these developing bodies, potentially impacting host cell metabolism.

Area of Science:

  • Virology
  • Cell Biology
  • Structural Biology

Background:

  • Spindle bodies (SBs) are key viral components in Heliothis armigera entomopoxvirus infections.
  • Understanding viral morphogenesis is crucial for controlling insect-borne diseases.

Purpose of the Study:

  • To elucidate the process of spindle body (SB) morphogenesis in Heliothis armigera entomopoxvirus.
  • To investigate the role of the major constituent protein (fusolin) and chaperone BiP in SB formation.

Main Methods:

  • Immunoelectron microscopy was employed to visualize the intricate process of SB assembly.
  • Analysis focused on the perinuclear space, endoplasmic reticulum (ER), and associated vesicles within infected cells.

Main Results:

  • SB morphogenesis involves iterative condensation, aggregation, and crystallization of fusolin.
  • The endoplasmic reticulum (ER)-specific chaperone BiP was found associated with SBs throughout their development.
  • Fusolin, the major protein component, undergoes a crystallization process during SB formation.

Conclusions:

  • SB formation is a complex, multi-step process involving protein crystallization within the ER.
  • The association of BiP with SBs suggests its sequestration, which may disrupt host cell metabolism.
  • Further research is needed to understand the implications of BiP sequestration on host-pathogen interactions.

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