Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Elongin from Saccharomyces cerevisiae.

C M Koth1, M V Botuyan, R J Moreland

  • 1Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 1A8.

The Journal of Biological Chemistry
|February 7, 2001
PubMed
Summary

Yeast elongin A and C form a complex essential for structural changes, though not required for cell viability. This complex did not enhance yeast transcription elongation by RNA polymerase II.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cancer blues? A promising judgment bias task indicates pessimism in nude mice with tumors.

Physiology & behavior·2021
Same author

Maternal effects obscure condition-dependent sex allocation in changing environments.

Royal Society open science·2019
Same author

Notes on Cases of Injury to the Skull and Its Contents.

Glasgow medical journal·2018
Same author

Blood pressure, arterial stiffness and exercise: does exercise increase the risk of acute cardiac events in older adults?

European journal of preventive cardiology·2018
Same author

Remarks on Sayre's Splint for Morbus Coxarius.

Edinburgh medical journal·2018
Same author

Notes on the State of Lithotomy, past and Present. From a Lecture.

Edinburgh medical journal·2018

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Elongin, a transcription elongation factor, comprises three subunits: elongin A, B, and C.
  • Homologues of elongin A and C, but not B, exist in yeast.
  • Yeast elongin A and C are non-essential for cell viability.

Purpose of the Study:

  • To investigate the interaction and structural properties of yeast elongin A and C.
  • To determine the role of the yeast elongin complex in transcription elongation.

Main Methods:

  • Purification of yeast elongin A and C complex.
  • Recombinant complex production in bacteria.
  • Limited proteolysis to identify interaction domains.
  • Circular dichroism and nuclear magnetic resonance spectroscopy for structural analysis.

Related Experiment Videos

Main Results:

  • Yeast elongin A and C can be purified as a complex.
  • The complex did not stimulate yeast RNA polymerase II-mediated transcription.
  • The N-terminal 144 residues of yeast elongin A mediate interaction with elongin C.
  • Yeast elongin A is unfolded but structurally modified upon binding elongin C.
  • Elongin C forms a stable dimer independently.

Conclusions:

  • Yeast elongin A and C form a stable complex with distinct structural properties.
  • The yeast elongin complex does not appear to function as a transcription elongation factor for RNA polymerase II.
  • Elongin C's dimerization and elongin A's structural changes highlight subunit-specific roles.