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Chiral separation by capillary electrochromatography.

G Gübitz1, M G Schmid

  • 1Institute of Pharmaceutical Chemistry, Karl-Franzens University of Graz, Austria. guebitz@kfunigraz.ac.at

Enantiomer
|April 14, 2000
PubMed
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This review covers advances in chiral capillary electrochromatography, detailing methods using chiral stationary phases, mobile phase additives, and coated open tubular capillaries. Emerging techniques like molecularly imprinted polymers and continuous beds are also highlighted for chiral separations.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Chiral capillary electrochromatography (CEC) is crucial for separating enantiomers.
  • Traditional methods involve packed capillaries or mobile phase additives.

Purpose of the Study:

  • To review the current state-of-the-art in chiral capillary electrochromatography.
  • To highlight recent advancements and emerging trends in the field.

Main Methods:

  • Review of packed capillary systems with chiral stationary phases (CSPs).
  • Analysis of achiral stationary phases with chiral selectors in the mobile phase.
  • Exploration of open tubular capillaries with wall-coated chiral selectors.
  • Discussion of molecularly imprinted polymers (MIPs) as CSPs.
  • Examination of polymeric continuous beds with incorporated chiral selectors.

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Main Results:

  • Various strategies effectively achieve chiral separations in CEC.
  • CSPs, mobile phase additives, and coated open tubular capillaries are established methods.
  • MIPs offer a challenging yet promising approach for chiral recognition.
  • Polymeric continuous beds represent a recent trend for enhanced chiral separations.

Conclusions:

  • Chiral capillary electrochromatography employs diverse stationary and mobile phase strategies for enantiomeric separation.
  • Emerging technologies like MIPs and continuous beds are advancing the capabilities of chiral CEC.