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Related Experiment Videos

Human Plasma Fibronectin Mediates Adhesion of U937 Cells by RGD and CS1.

Katoh1, Mohri, Ogawa

  • 1The First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan.

Journal of Thrombosis and Thrombolysis
|April 15, 2000
PubMed
Summary

Fibronectin adhesion to U937 cells involves specific integrin receptors, primarily alpha5beta1 and alpha4beta1, mediating interactions via RGDS and CS1 sequences. This clarifies mechanisms in cell adhesion and atherogenesis.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Immunology

Background:

  • Fibronectin is a key extracellular matrix protein involved in cell adhesion.
  • U937 cells are a human monocytic cell line frequently used in cell adhesion studies.
  • Specific domains and sequences within fibronectin mediate its interactions with cell surface receptors.

Purpose of the Study:

  • To elucidate the specific fibronectin binding mechanisms to U937 cells.
  • To identify the integrin receptors involved in this adhesion process.
  • To understand the roles of RGDS and CS1 sequences in fibronectin-U937 cell interactions.

Main Methods:

  • Dose-dependent binding assays of fibronectin to U937 cells.
  • Inhibition studies using RGDS, CS1 peptides, and fibronectin fragments.

Related Experiment Videos

  • Flow cytometry analysis for integrin expression on U937 cells.
  • Monoclonal antibody inhibition assays targeting specific integrin subunits.

    • Main Results:

    • Fibronectin binds U937 cells in a dose-dependent manner.
    • Adhesion is primarily mediated by the RGDS sequence binding to alpha5beta1 integrins and the CS1 sequence binding to alpha4beta1 integrins.
    • U937 cells express alpha3beta1, alpha4beta1, and alpha5beta1 integrins, but not alpha2beta1.
    • Monoclonal antibodies against alpha4beta1 and alpha5beta1 significantly inhibited U937 cell adhesion to fibronectin, with anti-alpha5beta1 being more potent.

    Conclusions:

    • The adhesion of U937 cells to fibronectin is specifically mediated by the RGDS and CS1 sequences interacting with alpha5beta1 and alpha4beta1 integrins, respectively.
    • These findings contribute to understanding the molecular mechanisms underlying cell adhesion, potentially relevant to tumor cell metastasis and atherogenesis.