Related Experiment Video
Updated: Jun 29, 2026
Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Published on: May 23, 2014
In vitro differentiation of human endometrial stromal cells: effect of transforming growth factor beta 1 on prolactin
H Celikkanat1, B Atac, K Piskin
1Dr. Zekai Tahir Burak Maternity Hospital, Ankara, Turkey.
Objective:
To investigate the role of Transforming Growth Factor beta 1 on differentiation of human endometrial stromal cells.
Study Design:
A prospective study.
Material And Methods:
Human endometrial cells obtained from 9 women were cultured in DMEM-HAMs F12 media to adequate cell confluence and then the flasks were cultured in the presence and absence of TGF b 1. The influence of TGF beta 1 were measured by prolactin production expressed as nanograms of prolactin/mg of total DNA in cells measured by diphenylamine reaction.
Results:
The levels of prolactin in the culture medium with and without TGF beta 1 were 0.16 +/- 0.27 ng/microgram DNA/day and 0.24 +/- 0.41 ng/microgram DNA/day respectively. There was no significant difference between groups.
Conclusion:
There is no direct effect of TGF beta 1 on differentiation of human endometrial stromal cells. However it may involve in more complex relationship in this process.
