Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Citrate utilization by homo- and heterofermentative lactobacilli.

R Medina de Figueroa1, F Alvarez, A Pesce de Ruiz Holgado

  • 1Centro de Referencia para Lactobacilos, Tucumán, Argentina. rfiguer@unt.edu.ar

Microbiological Research
|April 20, 2000
PubMed
Summary

Lactobacilli strains exhibit varied citrate utilization, with some using it as an energy source and others co-metabolizing it with glucose. All studied strains possess a citrate transport system inducible by citrate.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

(R)-Mandelonitrile Lyase, a Homolog of Pru du 10, Is a Major Peach Allergen.

Journal of investigational allergology & clinical immunology·2025
Same author

Comparative Passivation of Si(100) by H<sub>2</sub> and D<sub>2</sub> Atmospheres under Simultaneous Xe<sup>+</sup> Bombardment: An X-ray Photoelectron Spectroscopy Analysis.

Langmuir : the ACS journal of surfaces and colloids·2024
Same author

Prelacrimal window approach to the maxillary sinus: a systematic review and meta-analysis of the literature.

Rhinology·2024
Same author

The effects of gestational age on neonatal cholestasis: A retrospective cohort study.

Journal of neonatal-perinatal medicine·2024
Same author

R-mandelonitrile-lyase, homolog to Pru d 10, is a major peach allergen in peach allergic Spanish population.

Journal of investigational allergology & clinical immunology·2024
Same author

Corrigendum to "Menstrual cycle and exogenous attention toward emotional expressions" [Horm. Behav. 146 (2022) 105259].

Hormones and behavior·2023

Area of Science:

  • Microbiology
  • Food Science
  • Biochemistry

Background:

  • Lactobacilli are crucial in fermented foods and human health.
  • Understanding their metabolic capabilities, like citrate utilization, is key to optimizing fermentation processes and probiotic applications.
  • Citrate metabolism in lactobacilli can influence flavor profiles and nutrient availability.

Purpose of the Study:

  • To investigate and differentiate citrate utilization patterns among various lactobacilli strains.
  • To identify strains capable of using citrate as a sole energy source versus those that co-metabolize it.
  • To explore the inducibility and characteristics of the citrate transport system in lactobacilli.

Main Methods:

  • Culturing lactobacilli in Kempler and McKay and calcium citrate media, with and without glucose.

Related Experiment Videos

  • Analyzing growth and metabolic products to determine citrate utilization pathways.
  • Conducting citrate transport experiments and dot blot assays to investigate the citrate permease system.

    • Main Results:

    • Calcium citrate medium with glucose effectively distinguished citrate-fermenting lactobacilli.
    • Lactobacillus rhamnosus, Lactobacillus zeae, and Lactobacillus plantarum utilized citrate as their sole energy source.
    • Other strains co-metabolized glucose and citrate, with variable induction levels of the citrate transport system.
    • No equivalent plasmid coding for citrate permease was detected in the studied lactobacilli.

    Conclusions:

    • Citrate utilization is a strain-specific trait in lactobacilli, impacting their metabolic versatility.
    • The presence of an inducible citrate transport system is common, but its efficiency varies.
    • Citrate metabolism can contribute to the production of aroma compounds in fermented products.