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Reactions of heme peroxidases with peroxynitrite
1Institute of Applied Radiation Chemistry, Technical University of Lódź, Poland. lgebicka@mitr.p.lodz.pl
IUBMB Life
|April 20, 2000
Abstract:
The interaction of peroxynitrite, produced by ozonation of azide, with two heme peroxidases (horseradish peroxidase and lactoperoxidase) was studied. Enzymes retained full activity after incubation with peroxynitrite at neutral pH. Lactoperoxidase alone was found to catalyze peroxynitrite decomposition, whereas horseradish peroxidase accelerated peroxynitrite decomposition only in the presence of certain substrates. For example, in the presence of guaiacol the catalyzing effect was clear, but in the presence of trolox was only noticeable.