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Leukocyte adhesion and microvessel permeability.

P He1, J Wang, M Zeng

  • 1Department of Human Physiology, School of Medicine, University of California, Davis, California 95616, USA. pnhe@ucdavis.edu

American Journal of Physiology. Heart and Circulatory Physiology
|April 25, 2000
PubMed
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Leukocyte adhesion increases microvessel permeability. This effect is mediated by cAMP-dependent mechanisms and involves oxidants and mast cells, but is independent of leukocyte adhesion itself.

Area of Science:

  • Physiology
  • Immunology
  • Microcirculation Research

Background:

  • Leukocyte adhesion to microvessel walls is a key event in inflammation.
  • The direct impact of leukocyte adhesion on microvessel permeability remains incompletely understood.
  • Understanding these mechanisms is crucial for developing anti-inflammatory therapies.

Purpose of the Study:

  • To directly measure the effect of leukocyte adhesion on microvessel hydraulic conductivity (Lp).
  • To investigate the role of cyclic adenosine monophosphate (cAMP) in modulating this permeability increase.
  • To explore the involvement of oxidants and mast cells in leukocyte adhesion-induced permeability changes.

Main Methods:

  • Developed a method to measure Lp in individually perfused frog mesenteric venular microvessels.

Related Experiment Videos

  • Measured Lp before and after inducing leukocyte adhesion under controlled flow conditions.
  • Utilized isoproterenol to modulate cAMP levels, and ketotifen/desferrioxamine to assess oxidant and mast cell involvement.

    • Main Results:

    • Leukocyte adhesion significantly increased microvessel Lp (4.7-fold).
    • Isoproterenol prevented or reversed the Lp increase, indicating a cAMP-dependent mechanism.
    • Ketotifen and desferrioxamine attenuated the Lp increase, suggesting roles for oxidants and mast cells.

    Conclusions:

    • Leukocyte adhesion directly increases microvessel permeability via cAMP-dependent pathways.
    • These pathways are distinct from those regulating leukocyte adhesion itself.
    • Oxidants and mast cell activation contribute to leukocyte adhesion-induced increases in microvessel permeability.