Related Experiment Video
Updated: Oct 16, 2026

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
Published on: October 16, 2018
Vanadium-binding protein excreted by vanadate-reducing bacteria
A N Antipov1, N N Lyalikova, N P L'vov
1N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Abstract:
A vanadium-binding protein was isolated from the culture medium of the vanadium-reducing bacterium Pseudomonas isachenkovii by utilizing vanadate as the terminal electron acceptor upon anaerobic respiration. The protein was associated with vanadium at a molar ratio of approximately 1:20. It was purified to homogeneity and separated into three components by treatment with 1 M HCl followed by gel filtration: a protein, a vanadium-binding ligand, and inorganic vanadium. Electron paramagnetic resonance analysis showed that vanadium was associated with the protein in the 4+ oxidation state. The distribution of vanadium within the cell was studied by electron microscopy and x-ray microanalysis of P. isachenkovii cells. The results suggest that vanadium, accumulated in special swells on the surface of the cell membranes, is reduced and excreted to the medium.
Related Concept Videos
Microbial Bioremediation of Uranium
Drug Distribution: Plasma Protein Binding
ATP Driven Pumps III: V-type Pumps
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
Estimation of k and VD of Aminoglycosides
Negative Regulator Molecules

