Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intergenic complementation truncation mutants of cyclin-dependent kinase.

G A Bitter1, M M Tsai, A P Putzke

  • 1BitTech Inc., Agoura Hills, CA 91376-1499, USA. gab@bittech.net

Molecular & General Genetics : MGG
|April 25, 2000
PubMed
Summary

Researchers found that truncated human Cdk2 proteins can still regulate gene transcription in yeast by interacting with yeast proteins. This suggests that key functional domains of cyclin-dependent kinases (CDKs) are smaller than previously thought.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is passive shoulder external range influenced by body posture or shoulder abduction angle?

Shoulder & elbow·2026
Same author

Outcomes of exertional rhabdomyolysis following high-intensity resistance training.

Internal medicine journal·2016
Same author

The effects of iron supplementation in preweaning piglets.

Malaysian journal of nutrition·2012
Same author

Selectively transparent and conducting photonic crystal rear-contacts for thin-film silicon-based building integrated photovoltaics.

Optics express·2011
Same author

Acute medicine units: the current state of affairs in the North-West of England.

The journal of the Royal College of Physicians of Edinburgh·2010
Same author

Photonic crystal intermediate reflectors for micromorph solar cells: a comparative study.

Optics express·2010

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Protein Biochemistry

Background:

  • Saccharomyces cerevisiae genes PHO80 and PHO85 regulate PHO5 transcription via Pho4p phosphorylation.
  • Cyclin-dependent kinases (CDKs) are conserved regulatory proteins.
  • Previous studies showed functional hybrid human-yeast CDKs in yeast reporter gene assays.

Purpose of the Study:

  • To investigate the functional capacity of truncated human Cdk2-yeast Pho85p hybrid proteins.
  • To identify the minimal functional domains of human Cdk2 required for Pho85p activity in yeast.

Main Methods:

  • Utilized yeast genetics, including gene disruption and intergenic complementation assays.
  • Constructed and tested various truncated human Cdk2-yeast Pho85p hybrid proteins.

Related Experiment Videos

  • Assessed protein function via reporter gene assays and complementation of chromosomal mutations.

    • Main Results:

    • Truncated human Cdk2 proteins, even with significant C-terminal deletions, retained Pho85p function.
    • Functional hybrid CDKs were observed with Cdk2 proteins terminating after amino acid 90.
    • Truncations disrupted canonical ATP-binding and active sites but maintained Pho4p phosphorylation.
    • Functional truncations depended on the remaining pho85 coding region, suggesting fragment complementation.

    Conclusions:

    • Minimal domains of human Cdk2 are sufficient for Pho85p-mediated gene regulation in yeast.
    • Protein fragment complementation may explain the activity of truncated CDK hybrids.
    • These findings advance understanding of CDK structure-function relationships and conserved regulatory mechanisms.