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Positional effect of single bulge nucleotide on PNA(peptide nucleic acid)/DNA hybrid stability
N Sugimoto1, K Yamamoto, N Satoh
1Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Nucleic Acids Symposium Series
|April 26, 2000
Abstract:
We report positional effect of bulge nucleotide on PNA/DNA hybrid stability. CD spectra showed that PNA/DNA hybrids required at least seven base pairings at a stem region to form a bulged structure. On the other hand, DNA/DNA could form bulged structure when there are only four base pairings adjacent to the bulge nucleotide. We discuss why PNA requests such a many base pairings to form bulged structure from a nearest neighbor standpoint.