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A facile synthesis of DNA oligomers with modified backbones via the phosphoramidite method without nucleoside base
M Kataoka1, R Kawai, Y Hayakawa
1Graduate School of Human Informatics, Nagoya University, Chikusa, Japan.
Nucleic Acids Symposium Series
|April 26, 2000
Abstract:
A convenient synthesis of DNA oligomers with modified back bones including phosphorothioates and phosphotriesters has been developed on the basis of the phosphoramidite strategy without nucleoside base protection. In this synthesis, a new, convenient method for the preparation of N-free-5'-O-dimethoxytrityl-2'-deoxyribonucleoside by the direct O-selective dimethoxytritylation of the parent nucleosides has also been disclosed.