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Quantitative detection of a DNA ligase reaction on a quartz-crystal microbalance
Y Okahata1, Y Masunaga, H Matsuno
1Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Nucleic Acids Symposium Series
|April 26, 2000
Abstract:
We report here kinetic analyses of DNA ligation by using a DNA-immobilized quartz-crystal microbalance (QCM), which enables in situ real-time monitoring of both the binding of ligase and ligation reaction on DNA strands, as mass changes.