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Construction of nonnatural DNA library
1Division of Natural Environment and Bioorganic Chemistry, Faculty of Human Development and Sciences, Kobe University, Japan.
Nucleic Acids Symposium Series
|April 26, 2000
Abstract:
Nonnatural DNA polymerase substrates which contain many kinds of modified functional groups are synthesized. The C-5 position of dUTP was modified by amino acid, saccharide. These compounds were incorporated on a DNA double strand using E. Coli DNA polymerase. This nonnatural DNA library contains a larger diversity than native DNA or RNA and has a higher chemical stability than RNA. This library will be useful for in vitro selection study, combinatorial chemistry, and the preparation of supramolecular structures.