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Extended in vitro selection for synthesis of novel molecular recognition oligonucleotide derivatives
1Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Japan.
Nucleic Acids Symposium Series
|April 26, 2000
Abstract:
In vitro selection of RNA aptamer containing biotin-carrying nucleotide was carried out used for development of a new type of molecular sensor. Cytidine 5'-triphosphate (CTP) carrying biotinyl group at the N6-position was used in this technique. A pool of biotin-containing RNAs, which binds specifically to adenosine 5'-triphosphate (ATP), was obtained and used for competitive binding assay of ATP. The selected nonnatural RNA possesses many biotinyl groups to render it a high sensitivity toward ATP.