The functional analysis of 4.5S RNA in ribosomal translocation

4.5S RNA is a stable 114-nucleotide RNA of the bacterium Escherichia coli (E. coli). We found that 4.5S RNA have the ability of binding EF-G using gel mobility shift assay. Increasing in the concentration of GDP increase the binding activity of 4.5S RNA to EF-G. Based on these data, we propose that 4.5S RNA release EF-G from ribosome.