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High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
Published on: June 21, 2011
Simulation of the effect of magnification and image-rotation variations on three-dimensional reconstruction
1School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an, People's Republic of China. hbzhang@xjtu.edu.cn
Journal of Electron Microscopy
|May 3, 2000
Abstract:
Tilting a specimen may change the magnification and the image rotation for electron-microscopic images, because re-focusing with an objective lens is required to correct for the height variation of the tilted specimen. Computer simulation is performed to analyse the effect of variations in the magnification and the image rotation on the three-dimensional reconstruction in electron tomography.

