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Characterization and analysis of a new gene involved in glucose starvation response in Enterococcus faecalis
H Capiaux1, J C Giard, S Lemarinier
1Laboratoire de Microbiologie de l'Environnement, Institut de Recherche en Biologie Appliquée, Université de Caen, France.
International Journal of Food Microbiology
|May 3, 2000
Abstract:
The genome sequence of Enterococcus faecalis, led us to discover that gls24, encoding a general stress protein, seems to be in the second last position of a putative six-gene operon structure. Interestingly, another gene named orf4 located just upstream from gls24 shows strong identity (72%) with this last. To determine the role of the orf4 gene in E. faecalis, we have constructed a mutant strain by homologous recombination. Phenotypic analysis of these cells, reveals that Orf4 is probably involved in cell structure in stationary phase.