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NGF administered by microdialysis into rabbit vitreous
1Department of Ophthalmology, University Hospital of Lund, Sweden. Janina.Waga@oft.lu.se
Acta Ophthalmologica Scandinavica
|May 4, 2000
Summary
Microdialysis with novel polyether sulphone (PES) membranes enables intraocular administration of nerve growth factor (NGF) in acute rabbit eye experiments. This technique allows substance delivery without altering intraocular fluid balance.
Area of Science:
- Ophthalmology
- Neuroscience
- Biomaterials Science
Background:
- Microdialysis is a valuable technique in eye research, suitable for both chronic and acute substance administration and withdrawal.
- The method allows molecule passage across intraocular membranes without net fluid movement, preserving ocular homeostasis.
Purpose of the Study:
- To investigate the efficacy of microdialysis using a new type of high-permeability polyether sulphone (PES) membrane for intraocular drug delivery.
- To assess the ability of nerve growth factor (NGF) to permeate the PES membrane in the rabbit vitreous.
Main Methods:
- Acute microdialysis experiments were conducted in rabbit eyes.
- A high-permeability PES membrane (100,000 daltons cutoff) was implanted in the vitreous.
- The membrane was perfused with a solution containing 125I labeled NGF for varying durations, followed by radioactivity counting of the entire vitreous.
Main Results:
- Detectable levels of nerve growth factor (NGF), up to 10(-11)M, were found in the vitreous after perfusion.
- The results confirm the permeability of the PES membrane to NGF.
Conclusions:
- Microdialysis utilizing PES membranes is a viable method for the intraocular administration of NGF.
- This technique holds promise for targeted drug delivery in ocular research and treatment.