Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Grapevine fleck virus-like viruses in Vitis.

S Sabanadzovic1, N Abou-Ghanem, M A Castellano

  • 1Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy.

Archives of Virology
|May 5, 2000
PubMed
Summary

New degenerate primers enable specific detection of grapevine viruses, including Tymovirus and Marafivirus relatives. This research aids in identifying and understanding grapevine red globe virus (GRGV) and other related plant viruses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation.

Plant disease·2025
Same author

Re-examination of the Southern Hemisphere truffle genus <i>Amylascus</i> (<i>Pezizaceae, Ascomycota</i>) and characterization of the sister genus <i>Nothoamylascus</i> gen. nov.

Persoonia·2024
Same author

Three independent evolutionary events of sequestrate <i>Lactifluus</i> species in Australasia.

Fungal systematics and evolution·2022
Same author

<i>Hysterangiales</i> revisited: expanded phylogeny reveals new genera and two new suborders.

Fungal systematics and evolution·2022
Same author

Eight new <i>Elaphomyces</i> species (<i>Elaphomycetaceae, Eurotiales, Ascomycota</i>) from eastern North America.

Fungal systematics and evolution·2021
Same author

Three new black <i>Elaphomyces species</i> (<i>Elaphomycetaceae</i>, <i>Eurotiales</i>, <i>Ascomycota</i>) from eastern North America with notes on selected European species.

Fungal systematics and evolution·2020

Area of Science:

  • Plant Virology
  • Molecular Biology
  • Bioinformatics

Background:

  • Grapevine viruses pose significant threats to viticulture worldwide.
  • Accurate identification and phylogenetic analysis are crucial for disease management.
  • Existing methods may lack specificity or efficiency for certain grapevine viruses.

Purpose of the Study:

  • To design degenerate primers for amplifying specific genomic segments of Tymovirus and Marafivirus genera, and Grapevine fleck virus (GFkV).
  • To characterize previously unsequenced grapevine viruses, including the proposed Grapevine red globe virus (GRGV).
  • To establish phylogenetic relationships among these viruses and known tymoviruses/marafiviruses.

Main Methods:

  • Degenerate primer design based on available viral sequences.

Related Experiment Videos

  • Amplification, cloning, and sequencing of viral genome fragments (open reading frame 1).
  • Computer-assisted phylogenetic analysis and electron microscopy for ultrastructural modifications.
  • Reverse transcription-polymerase chain reaction (RT-PCR) for virus detection in grapevine sap.

    • Main Results:

    • Successful amplification and sequencing of genome portions from GFkV, Grapevine asteroid mosaic-associated virus (GAMaV), and the novel GRGV.
    • Phylogenetic analysis revealed close relationships between GFkV, GAMaV, GRGV, tymoviruses, and marafiviruses.
    • Ultrastructural analysis confirmed the relationship with tymoviruses.
    • Degenerate primers facilitated non-specific detection of the three viruses in crude grapevine sap.

    Conclusions:

    • The developed degenerate primers are effective for amplifying and detecting related grapevine viruses.
    • GFkV, GAMaV, and GRGV are phylogenetically linked to tymoviruses and marafiviruses.
    • RT-PCR using specific primers allows for precise identification of these grapevine viruses.