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Updated: Aug 15, 2026

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
Published on: July 28, 2010
Different types of response to foreign antigens by leech leukocytes
M de Eguileor1, A Grimaldi, G Tettamanti
1DBSF, University of Insubria, Varese, Italy. magda@mailserver.unimi.it
Abstract:
We used morphological and immunocytochemical approaches to characterize and to show the behavior of cells involved in leech inflammatory responses. Leeches were injected with bacterial lipopolysaccharide, fluoresceinated yeasts, sulfate spheres and ciliates (Protozoa). Shortly after injection, migrating cells appeared in the area of injection. The response of the cells occurred in relation to the injected micro or macro antigens. Each injection first provoked a migration of cells towards the non-self material. Afterwards, different responses (degranulation, phagocytosis, encapsulation, melanization) occurred. The migrating cells involved in these series of processes have a similar behavior and are characterized by CD markers of macrophages, NK cells and granulocytes, which are typical of many invertebrates and vertebrates.
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