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Recombinant expression of a selective blocker of M(1) muscarinic receptors
J Näsman1, M Jolkkonen, S Ammoun
1Department of Physiology, Uppsala University, BMC, Uppsala, S-751 23, Sweden. Johnny.Nasman@fysiologi.uu.se
Abstract:
Mamba venoms contain peptides with high selectivity for muscarinic receptors. Due to the limited availability of the M(1) muscarinic receptor-selective MT7 or m1-toxin 1, the peptide was expressed in Sf9 cells using a synthetic cDNA and purified. The isolated peptide had over four orders of magnitude higher affinity for the M(1) compared to M(2)-M(5) muscarinic receptors. The peptide strongly inhibited Ca(2+) mobilisation through recombinant and endogenously expressed M(1) receptors, having no effect on the function of the other subtypes. The MT7 peptide provides a unique tool for identification and functional characterisation of M(1) receptors in cells and tissues.
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