Blue dextran: influence on chromatographic profile and immunoreactivity of human follicle-stimulating hormone (HFSH)

When FSH is chromatographed on G-100 Sephadex in the absence of Blue Dextran, it elutes as a single peak in the region of monomeric FSH. The addition of Blue Dextran causes a significant portion of FSH, iodinated or uniodinated, to elute in the region of the void volume. When standard curves for FSH are performed under identical conditions, with tracer 125I-FSH from the void volume, and from the FSH monomer region, maximal immunoreactivity was significantly greater for the Blue Dextran-associated tracer.