Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alpha-antitrypsin Pi types in postmortem blood.

R C Talamo, W M Thurlbeck

    The American Review of Respiratory Disease
    |August 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Experimental Pulmonary Emphysema: The Effect of Intratracheal Injection of Cadmium Chloride Solution in the Guinea Pig.

    The American journal of pathology·2009
    Same author

    Expression of the insulin-like growth factor system in postpneumonectomy lung growth.

    Experimental lung research·1998
    Same author

    Comparative biochemistry of gestational and postnatal lung growth and development in the rat and human.

    Pediatric pulmonology·1997
    Same author

    Effect of triamcinolone acetonide on the development of the pulmonary airways in the fetal rat.

    Pediatric pulmonology·1997
    Same author

    Two different patterns of airway branching regulated by different components of the extracellular matrix in vitro.

    Experimental lung research·1996
    Same author

    Biochemical, clinical, and morphologic studies on lungs of infants with bronchopulmonary dysplasia.

    Pediatric pulmonology·1996

    Alpha-1 antitrypsin (AAT) Pi typing is reliable from postmortem serum, with identical results in paired samples. However, recent blood transfusions may interfere with typing accuracy.

    Area of Science:

    • Forensic Science
    • Clinical Chemistry
    • Genetics

    Background:

    • Alpha-1 antitrypsin (AAT) is a crucial proteinase inhibitor.
    • Accurate AAT Pi typing is essential for genetic and clinical studies.
    • Postmortem sample analysis presents unique challenges for biochemical typing.

    Purpose of the Study:

    • To evaluate the feasibility and reliability of determining alpha-1 antitrypsin (AAT) Pi types from postmortem serum.
    • To compare AAT Pi types from antemortem and postmortem samples.
    • To investigate the influence of pre-necropsy factors on AAT Pi typing.

    Main Methods:

    • Serum samples were collected at necropsy and compared with antemortem samples.
    • Alpha-1 antitrypsin (AAT) Pi typing was performed on paired samples.

    Related Experiment Videos

  • The impact of recent blood transfusions on serum typing was assessed.

    • Main Results:

    • Identical AAT Pi types were observed in 37 paired antemortem and postmortem serum samples.
    • Blood transfusions within 72 hours of death can render serum untypeable.
    • A high frequency of the Pis allele (0.074) was noted, potentially due to population genetics or postmortem alterations.

    Conclusions:

    • Postmortem serum is a viable source for alpha-1 antitrypsin (AAT) Pi typing.
    • Care must be taken to account for recent blood transfusions.
    • Further research in larger populations is recommended to clarify allele frequencies and potential associations with emphysema.