Related Experiment Videos
Establishment of monoclonal antibodies specific for Bacillus subtilis DB9011
1Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Japan.
Bioscience, Biotechnology, and Biochemistry
|May 10, 2000
Abstract:
Bacillus subtilis DB9011 is a strain with useful functions for agriculture. To establish a method for the discrimination of this strain from others, monoclonal antibodies (MAbs) were prepared. Although two established MAbs (MAb9B6 and MAb14D2) cross-react with some other Bacillus strains in ELISA, only B. subtilis DB9011 vegetative cells are recognized by both MAbs. MAb14D2 recognizes flagellin, a 34-kDa unit protein of flagella. The two MAbs established will provide powerful tools with which detailed analysis of this bacterial strain can be obtained under environmental conditions.