Related Experiment Video

Updated: Oct 16, 2026

Clinically Relevant Transplantable Tumor Models for Studying Metastatic Ovarian Cancer and Therapeutic Response in Mouse
10:45

Clinically Relevant Transplantable Tumor Models for Studying Metastatic Ovarian Cancer and Therapeutic Response in Mouse

Published on: May 19, 2026

Adenovirus-mediated transfer of a p53 gene in ovarian cancer

J Kigawa1, N Terakawa

  • 1Department of Obstetrics and Gynecology, Tottori University School of Medicine, Yonago, Japan.

Advances in Experimental Medicine and Biology
|May 16, 2000
PubMed

Insights

Investigational gene therapy using p53 gene transfer, specifically AxCAp53, shows promise for treating refractory ovarian cancer. Combining this with CDDP may overcome cisplatin resistance in ovarian cancer patients.

Area of Science:

  • Oncology
  • Gene Therapy
  • Molecular Biology

Background:

  • Recurrent or refractory ovarian cancer lacks effective conventional treatments.
  • Gene therapy, particularly p53 gene transfer, is an emerging modality for cancer treatment.
  • The p53 tumor suppressor gene plays a critical role in cell cycle regulation and apoptosis.

Purpose of the Study:

  • To evaluate the potential of a novel recombinant adenovirus carrying a wild-type p53 gene (AxCAp53) for ovarian cancer therapy.
  • To investigate the efficacy of AxCAp53 in combination with cisplatin (CDDP) in overcoming drug resistance.

Main Methods:

  • Development of a recombinant adenovirus vector, AxCAp53, for wild-type p53 gene delivery.
  • Assessment of AxCAp53's efficacy in suppressing ovarian cancer cell growth.
  • Evaluation of AxCAp53's ability to sensitize p53-deficient ovarian cancer cells to CDDP.

Main Results:

  • AxCAp53 alone demonstrated insufficient efficacy in suppressing ovarian cancer cell growth.
  • AxCAp53 significantly increased the sensitivity of p53-deleted ovarian cancer cells to CDDP.
  • The combination therapy showed potential in overcoming CDDP resistance.

Conclusions:

  • While AxCAp53 monotherapy has limitations, its combination with CDDP presents a promising strategy for treating cisplatin-resistant ovarian cancer.
  • Investigational gene therapy protocols involving p53 gene transfer warrant consideration for patients with refractory ovarian cancer.

More Related Videos

Transplantation Into the Mouse Ovarian Fat Pad09:25

Transplantation Into the Mouse Ovarian Fat Pad

Published on: September 7, 2016

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts10:27

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Published on: July 25, 2020

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...