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Updated: Oct 16, 2026

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
Linear free energy relationships implicate three modes of binding for fluoroaromatic inhibitors to a mutant of
J B Doyon1, E A Hansen, C Y Kim
1Department of Chemistry, Swarthmore College, Pennsylvania 19081, USA.
Abstract:
[figure: see text] Linear free energy relationships between binding affinity and hydrophobicity for a library of fluoroaromatic inhibitors of F131V carbonic anhydrase II (CA) implicate three modes of interaction. X-ray crystal structures suggest that F131 interacts with fluoroaromatic inhibitors, while P202, on the opposite side of the active site cleft, serves as the site of the hydrophobic contact in the case of the F131V mutant. 2-Fluorinated compounds bind more tightly, perhaps due to the field effect of the nearby fluorine on the acidity of the amide proton.
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