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Rosette formation with mouse erythrocytes: probable marker for human B lymphocytes
International Archives of Allergy and Applied Immunology
|January 1, 1975
Summary
Mouse red blood cells (MRBC) can identify B lymphocytes. Increased MRBC rosettes correlated with B cell markers in patients with chronic lymphocytic leukemia, suggesting MRBC as a potential B cell marker.
Area of Science:
- Immunology
- Hematology
Background:
- Peripheral blood lymphocytes play crucial roles in immune responses.
- Hematological disorders involve abnormalities in blood cell formation and function.
Purpose of the Study:
- To investigate the potential of mouse red blood cells (MRBC) as a marker for B lymphocytes.
- To evaluate MRFC formation in healthy controls and patients with hematological disorders.
Main Methods:
- Peripheral blood lymphocytes from 10 healthy controls and 15 patients were analyzed.
- Spontaneous formation of mouse red blood cell-forming rosettes (MRFC) was assessed.
- Rosette formation with sheep red blood cells (SRFC) and complement-coated sheep erythrocytes (EAC rosettes) was also evaluated.
Main Results:
- Healthy controls showed 8.1% MRFC.
- Five patients with chronic lymphocytic leukemia exhibited increased MRFC, paralleling EAC rosette increases.
- One lymphoma patient with elevated SRFC did not show increased MRFC.
Conclusions:
- Mouse red blood cells (MRBC) show promise as a B cell marker.
- MRFC formation may aid in identifying B lymphocytes, particularly in chronic lymphocytic leukemia.
- MRBC could serve as an additional detection marker for B lymphocytes through spontaneous rosette formation.