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Related Experiment Videos

Developmental changes in capacitative Ca(2+) entry in mouse mammary epithelial cells.

J Ichikawa1, K Furuya, S Miyata

  • 1Kyoto Institute of Technology, Department of Applied Biology, Matsugasaki, Sakyo-ku, Japan. a8820004@ipc.kit.ac.jp

Cell Biochemistry and Function
|May 18, 2000
PubMed
Summary

Capacitative calcium (Ca2+) entry in mouse mammary cells peaks early in pregnancy and declines as pregnancy progresses. Calcium release from stores remains constant, suggesting Ca2+ entry is key to mammary development.

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Area of Science:

  • Cell Biology
  • Physiology
  • Reproductive Biology

Background:

  • Mammary gland development during pregnancy involves complex cellular processes.
  • Calcium ions (Ca2+) play critical roles in cellular signaling and development.
  • Understanding calcium dynamics is crucial for comprehending pregnancy-induced mammary changes.

Purpose of the Study:

  • To investigate developmental changes in capacitative Ca2+ entry and Ca2+ release from intracellular stores.
  • To correlate these calcium dynamics with the progression of pregnancy in mouse mammary epithelial cells.

Main Methods:

  • Utilized the fura-2 fluorescence method to measure intracellular Ca2+ concentrations.
  • Assessed Ca2+ release using thapsigargin in a Ca2+-free solution.
  • Measured capacitative Ca2+ entry by re-adding extracellular Ca2+ after store depletion.

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Main Results:

  • Capacitative Ca2+ entry was highest on day 3 of pregnancy and decreased as pregnancy advanced.
  • Ca2+ release from intracellular stores remained unchanged throughout all measured developmental stages.
  • A strong correlation was observed between capacitative Ca2+ entry and pregnancy-induced mammary development.

Conclusions:

  • Capacitative Ca2+ entry, but not Ca2+ release, is closely linked to mammary epithelial cell development during pregnancy.
  • These findings highlight the specific role of capacitative Ca2+ entry in mediating pregnancy-induced mammary gland changes.