Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detection of chemically induced DNA damage by derivative square wave voltammetry.

J Mbindyo1, L Zhou, Z Zhang

  • 1Department of Chemistry, University of Connecticut, Storrs 06269-3060, USA.

Analytical Chemistry
|May 18, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated 3D-printed unibody immunoarray for chemiluminescence detection of cancer biomarker proteins.

Lab on a chip·2017
Same author

Bioconjugation of Antibodies and Enzyme Labels onto Magnetic Beads.

Methods in enzymology·2016
Same author

Paper-based electrochemical immunoassay for rapid, inexpensive cancer biomarker protein detection.

Analytical methods : advancing methods and applications·2014
Same author

Formal reduction potentials and redox chemistry of polyhalogenated biphenyls in a bicontinuous microemulsion.

Environmental science & technology·2011
Same author

Borohydride, micellar, and exciplex-enhanced dechlorination of chlorobiphenyls.

Environmental science & technology·2011
Same author

Dechlorination of polychlorinated biphenyls on soils and clay by electrolysis in a bicontinuous microemulsion.

Environmental science & technology·2011
Same journal

Machine Learning-Assisted Nanopore for Enhanced Fingerprinting Analysis of Functional Glycans.

Analytical chemistry·2026
Same journal

Correction to "Maleylpyruvic Acid-Inducible Gene Expression System and Its Application for the Development of Gentisic Acid Biosensor".

Analytical chemistry·2026
Same journal

Computer-Aided Rational Hapten Design for Broad-Spectrum Monoclonal Antibody Development against Anthraquinones and Its Application in Lateral Flow Immunoassay.

Analytical chemistry·2026
Same journal

One-Step Chemoenzymatic Labeling and Oxime-Reversible Enrichment for O-GlcNAcylation Profiling under Oxidative Stress.

Analytical chemistry·2026
Same journal

Acid/NIR Dual-Responsive Nanoplatform with AND Logic-Gated Controlled Nitric Oxide Release for Companion Theranostics of Tumors.

Analytical chemistry·2026
Same journal

Multicharged Foldable Plasma Membrane Probes for Precise Cancer Cell Discrimination and Fluorescence-Guided Surgery.

Analytical chemistry·2026
See all related articles

This study shows how styrene oxide damages DNA films. Derivative square wave voltammetry detected DNA base oxidation, with DNA/AQ films offering the best signal for monitoring this chemical damage.

Area of Science:

  • Electrochemistry
  • Biomaterials Science
  • Analytical Chemistry

Background:

  • DNA damage can occur through reactions with environmental toxins.
  • Electrochemical methods offer sensitive detection of DNA modifications.
  • Developing robust DNA-based sensors requires stable film formats.

Purpose of the Study:

  • To investigate DNA film damage induced by styrene oxide.
  • To evaluate electrochemical detection of DNA base oxidation.
  • To compare different DNA film preparation methods for sensing applications.

Main Methods:

  • Preparation of double-stranded DNA films on pyrolytic graphite electrodes using Eastman AQ38S or covalent binding.
  • Incubation of DNA films with styrene oxide.
  • Detection of DNA damage using derivative square wave voltammetry.

Related Experiment Videos

  • Confirmation of DNA damage via capillary electrophoresis.

    • Main Results:

    • DNA films exhibited characteristic oxidation peaks for DNA bases after styrene oxide exposure.
    • DNA/AQ films demonstrated superior signal-to-background ratios compared to covalently bound films.
    • Increased incubation time with styrene oxide correlated with higher oxidation peak integrals.
    • The susceptibility to damage varied among different types of double-stranded DNA.

    Conclusions:

    • Derivative square wave voltammetry is effective for detecting styrene oxide-induced DNA damage in films.
    • DNA/AQ films provide a promising platform for electrochemical sensing of DNA damage.
    • The extent of DNA damage is dependent on incubation time and DNA source.