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Circular dichroism as a detection method in the screening of enantioselective catalysts
M T Reetz1, K M Kuhling, H Hinrichs
1Max-Planck-Institut fur Kohlenforschung, Mulheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de
Chirality
|May 29, 2000
Abstract:
The combination of liquid chromatography (HPLC), UV/Vis-spectroscopy and circular dichroism (CD) can be used to construct a high-throughput screening system to determine the enantioselectivity of enzyme- or metal-catalyzed reduction of acetophenone with formation of (S)- and (R)-1-phenylethanol. Prerequisite for the viability of this system is the experimental finding that the anisotropy factor g is linearly related to the enantiomeric excess (ee) and that it is independent of concentration, thereby excluding possible aggregation effects.